Artemia Cysts Hatching & Incubation Manual
A step-by-step scientific guide to achieving ≥ 85% hatch-out rates, maximum nauplii biomass, and clean separation for marine larvae.
Optimal Hatching Parameters
Achieving consistent high hatching efficiency requires strict control of environmental factors in conical hatching tanks.
Salinity
Use natural seawater or synthetic sea salt. Low salinity prolongs hatching time.
Water Temperature
Maintain constant temperature. Temperatures below 25 °C drastically decrease hatch-out velocity.
Continuous Aeration
Keep cysts in continuous suspension. Avoid dead zones where cysts settle.
Light Intensity
Light triggers the embryonic development mechanism during the first 2 hours of hydration.
Optimal Hatching Conical Tank Dynamics
Maintains osmotic balance for rapid embryo hydration.
Ensures synchronized 20–24 hr hatch-out velocity.
Prevents cyst sedimentation and dead zones in tank corners.
4-Step Hatchery Operating Procedure
Disinfection & Pre-hydration
Pre-soak dry Artemia cysts in clean freshwater for 30 minutes at 20–25 °C before transferring to salt water.
Conical Tank Incubation
Fill conical hatching vessel with 30 ppt saltwater. Add cysts at a maximum density of 2 grams per liter.
Harvesting & Separation
Turn off aeration after 20–24 hours. Nauplii swim toward light at the bottom while empty shells float to top.
Cold-Storage Enrichment
Rinse harvested nauplii thoroughly. Store in chilled seawater (4–6 °C) or enrich with Emerald Chlorella.